PrimePCR™ Probe Assay: RGD1563045, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RGD1563045, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGD1563045, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037535
Assay Design:   exonic
Chromosome Location:   7:79160073-79160237question
Amplicon Length:   135
Splice Variants Targeted:   ENSRNOT00000029007 ENSRNOT00000046525 ENSRNOT00000036333 ENSRNOT00000048646 ENSRNOT00000037123 ENSRNOT00000006485 ENSRNOT00000009816 ENSRNOT00000049151 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000017542 ENSRNOT00000016409 ENSRNOT00000045509 ENSRNOT00000064857 ENSRNOT00000019331 ENSRNOT00000013313 ENSRNOT00000071898 ENSRNOT00000052141 ENSRNOT00000020513 ENSRNOT00000050690 ENSRNOT00000041103 ENSRNOT00000031545 ENSRNOT00000046365 ENSRNOT00000047694 ENSRNOT00000039882 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1563045
Gene Name:   Protein RGD1563045
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000029130
Entrez:   500859
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.230000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download