PrimePCR™ Probe Assay: RGD1562929, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037460
Assay Design:   exonic
Chromosome Location:   9:117331858-117331971question
Amplicon Length:   84
Splice Variants Targeted:   ENSRNOT00000074210 ENSRNOT00000001265 ENSRNOT00000016316 ENSRNOT00000060007 ENSRNOT00000046621 ENSRNOT00000044452 ENSRNOT00000017235 ENSRNOT00000016538 ENSRNOT00000012902 ENSRNOT00000072956 ENSRNOT00000008071 ENSRNOT00000013733 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000071210 ENSRNOT00000049138 ENSRNOT00000050803 ENSRNOT00000071995 ENSRNOT00000050291 ENSRNOT00000042619 ENSRNOT00000074618 ENSRNOT00000049571 ENSRNOT00000048356 ENSRNOT00000050384 ENSRNOT00000048123 ENSRNOT00000054860 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1562929
Gene Name:   Protein RGD1562929
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050799
Entrez:   316745
UniGene:   Not Available
Chromosome Mapping:   9q38

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.460000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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