PrimePCR™ Probe Assay: Gm8325, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037374
Assay Design:   exonic
Chromosome Location:   9:40490075-40490194question
Amplicon Length:   90
Splice Variants Targeted:   ENSRNOT00000037392 ENSRNOT00000031401 ENSRNOT00000058387 ENSRNOT00000026844 ENSRNOT00000032158 ENSRNOT00000068528 ENSRNOT00000035048 ENSRNOT00000040850

Gene Information

Description Not Available

Gene Symbol:   Gm8325
Gene Name:   Predicted pseudogene 8325
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000022456
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.750000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download