PrimePCR™ Probe Assay: Trim71, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037325
Assay Design:   exonic
Chromosome Location:   8:121865977-121866114question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000047198

Gene Information

Description Not Available

Gene Symbol:   Trim71
Gene Name:   Tripartite motif-containing protein 71
Aliases:   Not Available
RefSeq:   NM_001191801
Ensembl:   ENSRNOG00000030098
Entrez:   301042
UniGene:   Rn.225076
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.720000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download