This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Slc30a2, Rat
PrimePCR™ Template for Probe Assay: Slc30a2, Rat
The protein encoded by this gene is a zinc transporter. It is localized in the vesicles and suggested to protect the cells from zinc toxicity by facilitating vesicular sequestration. Alternatively spliced transcript variants have been found for this gene, one of which initiates translation from a non-AUG (CUG) start codon. [provided by RefSeq, Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.