PrimePCR™ Probe Assay: Cst12, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0037001
Assay Design:   exonic
Chromosome Location:   3:149528628-149528765question
Amplicon Length:   108
Splice Variants Targeted:   ENSRNOT00000006608

Gene Information

member of the male reproductive subgroup of the cystatin type 2 family of cysteine proteinase inhibitors [RGD, Feb 2006]

Gene Symbol:   Cst12
Gene Name:   Cystatin-12 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000004946
Entrez:   266776
UniGene:   Rn.58124
Chromosome Mapping:   3q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.720000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download