PrimePCR™ Probe Assay: RT1-S2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RT1-S2, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RT1-S2, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0036822
Assay Design:   exonic
Chromosome Location:   20:5254337-5254448question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000048858 ENSRNOT00000000990 ENSRNOT00000052311 ENSRNOT00000040634 ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000039155 ENSRNOT00000038725 ENSRNOT00000042866 ENSRNOT00000050246 ENSRNOT00000074123 ENSRNOT00000071931 ENSRNOT00000061415 ENSRNOT00000048535 ENSRNOT00000061395 ENSRNOT00000036147 ENSRNOT00000050159 ENSRNOT00000042882 ENSRNOT00000048036 ENSRNOT00000001035 ENSRNOT00000001036 ENSRNOT00000048076 ENSRNOT00000044520 ENSRNOT00000049540 ENSRNOT00000061114 ENSRNOT00000047527 ENSRNO

Gene Information

may play a role in antigen presentation [RGD, Feb 2006]

Gene Symbol:   RT1-S2
Gene Name:   RT1 class Ib, locus S2 precursor
Aliases:   Not Available
RefSeq:   NM_001008857
Ensembl:   ENSRNOG00000029001
Entrez:   24994
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.980000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download