PrimePCR™ Probe Assay: Hus1b, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0036186
Assay Design:   exonic
Chromosome Location:   17:36739067-36739181question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000039177 ENSRNOT00000016797 ENSRNOT00000040373 ENSRNOT00000022534 ENSRNOT00000047960 ENSRNOT00000045913 ENSRNOT00000043854 ENSRNOT00000074097 ENSRNOT00000072505 ENSRNOT00000009898

Gene Information

Description Not Available

Gene Symbol:   Hus1b
Gene Name:   Checkpoint protein HUS1B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049323
Entrez:   691382
UniGene:   Rn.204369
Chromosome Mapping:   17p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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