PrimePCR™ Probe Assay: RGD1564370, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RGD1564370, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGD1564370, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0035572
Assay Design:   exonic
Chromosome Location:   20:44569495-44569633question
Amplicon Length:   109
Splice Variants Targeted:   ENSRNOT00000007828 ENSRNOT00000068181 ENSRNOT00000029007 ENSRNOT00000037123 ENSRNOT00000006485 ENSRNOT00000066773 ENSRNOT00000007949 ENSRNOT00000009816 ENSRNOT00000048063 ENSRNOT00000049151 ENSRNOT00000049436 ENSRNOT00000006754 ENSRNOT00000044551 ENSRNOT00000014590 ENSRNOT00000017542 ENSRNOT00000073389 ENSRNOT00000056722 ENSRNOT00000024445 ENSRNOT00000045509 ENSRNOT00000027232 ENSRNOT00000013435 ENSRNOT00000014395 ENSRNOT00000000052 ENSRNOT00000068168 ENSRNOT00000011601 ENSRNOT00000064857 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1564370
Gene Name:   Protein RGD1564370
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000033652
Entrez:   499457
Chromosome Mapping:   20q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.370000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download