PrimePCR™ Probe Assay: Krtap1-1, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0035407
Assay Design:   exonic
Chromosome Location:   10:87298726-87298844question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000074700 ENSRNOT00000071214 ENSRNOT00000019356 ENSRNOT00000030823 ENSRNOT00000032739 ENSRNOT00000004486 ENSRNOT00000043951 ENSRNOT00000045946 ENSRNOT00000067913 ENSRNOT00000013398 ENSRNOT00000013428 ENSRNOT00000004365 ENSRNOT00000073560 ENSRNOT00000016924 ENSRNOT00000064320 ENSRNOT00000002535 ENSRNOT00000041345 ENSRNOT00000075047 ENSRNOT00000025808 ENSRNOT00000047459 ENSRNOT00000037980 ENSRNOT00000050087 ENSRNOT00000074097 ENSRNOT00000072505

Gene Information

Description Not Available

Gene Symbol:   Krtap1-1
Gene Name:   Protein LOC100361571
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000012955
Entrez:   100361571
UniGene:   Not Available
Chromosome Mapping:   10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 37.590000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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