PrimePCR™ Probe Assay: Nkx1-1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Nkx1-1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Nkx1-1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0035263
Assay Design:   exonic
Chromosome Location:   14:83290547-83290700question
Amplicon Length:   124
Splice Variants Targeted:   ENSRNOT00000042692 ENSRNOT00000041244 ENSRNOT00000061907 ENSRNOT00000041532 ENSRNOT00000075386 ENSRNOT00000073485 ENSRNOT00000046048 ENSRNOT00000017854 ENSRNOT00000000672 ENSRNOT00000006526 ENSRNOT00000004911 ENSRNOT00000056145 ENSRNOT00000067416 ENSRNOT00000055597 ENSRNOT00000011213 ENSRNOT00000055427 ENSRNOT00000009101 ENSRNOT00000073177 ENSRNOT00000057911 ENSRNOT00000016281 ENSRNOT00000022904 ENSRNOT00000074875 ENSRNOT00000074847

Gene Information

Description Not Available

Gene Symbol:   Nkx1-1
Gene Name:   Protein Nkx1-1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000005113
Entrez:   298981
Chromosome Mapping:   14q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.140000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download