PrimePCR™ Probe Assay: RT1-M6-2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0034973
Assay Design:   exonic
Chromosome Location:   20:4113962-4114078question
Amplicon Length:   87
Splice Variants Targeted:   ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000032992 ENSRNOT00000011787 ENSRNOT00000010043

Gene Information

Description Not Available

Gene Symbol:   RT1-M6-2
Gene Name:   RT1 class I, locus M6, gene 2 precursor
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000040052
Entrez:   365527
UniGene:   Rn.163419
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.810000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download