PrimePCR™ Probe Assay: Hist1h2ail1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist1h2ail1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist1h2ail1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0034370
Assay Design:   exonic
Chromosome Location:   17:45671931-45672038question
Amplicon Length:   78
Splice Variants Targeted:   ENSRNOT00000021362 ENSRNOT00000008328 ENSRNOT00000004329 ENSRNOT00000050223 ENSRNOT00000072269 ENSRNOT00000068710 ENSRNOT00000072019 ENSRNOT00000074292 ENSRNOT00000072119 ENSRNOT00000074024 ENSRNOT00000074763 ENSRNOT00000073188 ENSRNOT00000073901 ENSRNOT00000075332 ENSRNOT00000042805 ENSRNOT00000052073 ENSRNOT00000049001 ENSRNOT00000040906

Gene Information

Description Not Available

Gene Symbol:   Hist1h2ail1
Gene Name:   Histone H3.1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046434
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.130000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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