PrimePCR™ Probe Assay: 9330160F10Rik, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: 9330160F10Rik, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: 9330160F10Rik, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0034302
Assay Design:   exonic
Chromosome Location:   10:55385423-55385567question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000021037 ENSRNOT00000035915 ENSRNOT00000060404 ENSRNOT00000024831 ENSRNOT00000027847 ENSRNOT00000009936 ENSRNOT00000010333 ENSRNOT00000041689 ENSRNOT00000050649 ENSRNOT00000058007 ENSRNOT00000037941 ENSRNOT00000008679 ENSRNOT00000016714 ENSRNOT00000057977 ENSRNOT00000033975 ENSRNOT00000017461 ENSRNOT00000019502 ENSRNOT00000006443 ENSRNOT00000022384 ENSRNOT00000008507 ENSRNOT00000057157

Gene Information

Description Not Available

Gene Symbol:   9330160F10Rik
Gene Name:   RIKEN cDNA 9330160F10 gene
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000037672
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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