PrimePCR™ Probe Assay: RGD1563579, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0034174
Assay Design:   exonic
Chromosome Location:   X:82032456-82032561question
Amplicon Length:   76
Splice Variants Targeted:   ENSRNOT00000011793 ENSRNOT00000034681 ENSRNOT00000039250 ENSRNOT00000032240 ENSRNOT00000032995 ENSRNOT00000012377 ENSRNOT00000016555 ENSRNOT00000009205 ENSRNOT00000038120 ENSRNOT00000030319 ENSRNOT00000037416 ENSRNOT00000014849 ENSRNOT00000048888 ENSRNOT00000050117 ENSRNOT00000057412 ENSRNOT00000042570 ENSRNOT00000051668 ENSRNOT00000047001 ENSRNOT00000050331 ENSRNOT00000043027 ENSRNOT00000049689

Gene Information

Description Not Available

Gene Symbol:   RGD1563579
Gene Name:   Protein RGD1563579
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045666
Entrez:   367874
UniGene:   Not Available
Chromosome Mapping:   Xq31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.220000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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