PrimePCR™ Probe Assay: Hist1h4m, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Hist1h4m, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Hist1h4m, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033891
Assay Design:   exonic
Chromosome Location:   17:58650596-58650714question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000015307 ENSRNOT00000034102 ENSRNOT00000035317 ENSRNOT00000051467 ENSRNOT00000043151 ENSRNOT00000047303 ENSRNOT00000074900 ENSRNOT00000015200 ENSRNOT00000052087 ENSRNOT00000071770 ENSRNOT00000075065 ENSRNOT00000074109 ENSRNOT00000072286 ENSRNOT00000071518 ENSRNOT00000075028 ENSRNOT00000040210

Gene Information

Description Not Available

Gene Symbol:   Hist1h4m
Gene Name:   Histone H4
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046900
Entrez:   291152
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.540000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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