PrimePCR™ Probe Assay: Krtap3-3l1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033842
Assay Design:   exonic
Chromosome Location:   10:87271665-87271796question
Amplicon Length:   102
Splice Variants Targeted:   ENSRNOT00000038449 ENSRNOT00000002692 ENSRNOT00000044378 ENSRNOT00000023140 ENSRNOT00000063882 ENSRNOT00000059602 ENSRNOT00000029041 ENSRNOT00000014791 ENSRNOT00000027829 ENSRNOT00000009825 ENSRNOT00000073863 ENSRNOT00000064083 ENSRNOT00000028008 ENSRNOT00000068156 ENSRNOT00000024679 ENSRNOT00000031796 ENSRNOT00000049895 ENSRNOT00000032248 ENSRNOT00000005903 ENSRNOT00000052087 ENSRNOT00000014129 ENSRNOT00000055822 ENSRNOT00000065288 ENSRNOT00000039983 ENSRNOT00000045189

Gene Information

Description Not Available

Gene Symbol:   Krtap3-3l1
Gene Name:   Protein Krtap3-3; Protein Krtap3-3l1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049383
Entrez:   680060
UniGene:   Not Available
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996900
y-intercept 37.010000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download