PrimePCR™ Probe Assay: Pigm, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033642
Assay Design:   exonic
Chromosome Location:   13:95282485-95282587question
Amplicon Length:   73
Splice Variants Targeted:   ENSRNOT00000010551

Gene Information

mannosyltransferase that catalyzes the transfer of the first mannose to glycosylphosphatidylinositol in GPI anchor biosynthesis; activity may be localized to the lumenal side of the endoplasmic reticulum membrane [RGD, Feb 2006]

Gene Symbol:   Pigm
Gene Name:   GPI mannosyltransferase 1
Aliases:   Not Available
RefSeq:   NM_024144
Ensembl:   ENSRNOG00000007735
Entrez:   79112
UniGene:   Rn.163231
Chromosome Mapping:   13q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 37.040000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download