PrimePCR™ Probe Assay: Gm4793, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Gm4793, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Gm4793, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033367
Assay Design:   exonic
Chromosome Location:   13:57463073-57463226question
Amplicon Length:   124
Splice Variants Targeted:   ENSRNOT00000068150 ENSRNOT00000048109 ENSRNOT00000010242 ENSRNOT00000049759 ENSRNOT00000072708 ENSRNOT00000021621 ENSRNOT00000022328 ENSRNOT00000065234 ENSRNOT00000008637 ENSRNOT00000007608 ENSRNOT00000044477 ENSRNOT00000016440 ENSRNOT00000048300 ENSRNOT00000039411 ENSRNOT00000014921 ENSRNOT00000011287 ENSRNOT00000023488 ENSRNOT00000017386 ENSRNOT00000020711 ENSRNOT00000051058 ENSRNOT00000035157 ENSRNOT00000016752

Gene Information

Description Not Available

Gene Symbol:   Gm4793
Gene Name:   Predicted gene 4793
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000026400
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.991600
y-intercept 37.550000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
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3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download