PrimePCR™ Probe Assay: Spetex-2H, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033317
Assay Design:   exonic
Chromosome Location:   15:9591385-9591496question
Amplicon Length:   82
Splice Variants Targeted:   ENSRNOT00000043153 ENSRNOT00000061525 ENSRNOT00000042743 ENSRNOT00000049746 ENSRNOT00000051066 ENSRNOT00000073775 ENSRNOT00000071982 ENSRNOT00000043269 ENSRNOT00000073673 ENSRNOT00000074599 ENSRNOT00000071620 ENSRNOT00000061476 ENSRNOT00000073828 ENSRNOT00000051488 ENSRNOT00000073169 ENSRNOT00000041304 ENSRNOT00000074550 ENSRNOT00000048862 ENSRNOT00000071886 ENSRNOT00000050485 ENSRNOT00000075578 ENSRNOT00000045515 ENSRNOT00000038077 ENSRNOT00000024165 ENSRNOT00000072533 ENSRNOT00000048256

Gene Information

Description Not Available

Gene Symbol:   Spetex-2H
Gene Name:   Spetex-2H protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032043
Entrez:   361008
UniGene:   Rn.162039
Chromosome Mapping:   15p16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.600000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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