PrimePCR™ Probe Assay: Olr1248, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033072
Assay Design:   exonic
Chromosome Location:   4:1684307-1684492question
Amplicon Length:   156
Splice Variants Targeted:   ENSRNOT00000073368 ENSRNOT00000071683 ENSRNOT00000072371 ENSRNOT00000072622 ENSRNOT00000072751 ENSRNOT00000074928 ENSRNOT00000073888 ENSRNOT00000049094 ENSRNOT00000072964 ENSRNOT00000073505 ENSRNOT00000071502 ENSRNOT00000073941 ENSRNOT00000044273 ENSRNOT00000050849 ENSRNOT00000049093 ENSRNOT00000016674 ENSRNOT00000074056 ENSRNOT00000074742 ENSRNOT00000073159 ENSRNOT00000075251 ENSRNOT00000075191 ENSRNOT00000072517 ENSRNOT00000075359 ENSRNOT00000073523 ENSRNOT00000071676 ENSRNOT00000074022 ENSRNO

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1248
Gene Name:   Olfactory receptor Olr1248
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050945
Entrez:   300573
UniGene:   Rn.143522
Chromosome Mapping:   4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download