PrimePCR™ Probe Assay: Hist2h3c2, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0033038
Assay Design:   exonic
Chromosome Location:   17:45488276-45488396question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000045099 ENSRNOT00000038203 ENSRNOT00000027315 ENSRNOT00000065048 ENSRNOT00000045937 ENSRNOT00000018009 ENSRNOT00000057977 ENSRNOT00000048446 ENSRNOT00000066774 ENSRNOT00000033196 ENSRNOT00000048543 ENSRNOT00000050223 ENSRNOT00000054927 ENSRNOT00000036646 ENSRNOT00000057073 ENSRNOT00000047848 ENSRNOT00000049681 ENSRNOT00000068710 ENSRNOT00000072019 ENSRNOT00000074292 ENSRNOT00000072119 ENSRNOT00000074024 ENSRNOT00000074763 ENSRNOT00000073188 ENSRNOT00000073901 ENSRNOT00000075332 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Hist2h3c2
Gene Name:   Histone H3
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045646
Entrez:   310678
UniGene:   Rn.218658
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.340000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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