PrimePCR™ Probe Assay: SAP18, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032646
Assay Design:   exonic
Chromosome Location:   10:89432269-89432358question
Amplicon Length:   60
Splice Variants Targeted:   ENSRNOT00000025377 ENSRNOT00000014397 ENSRNOT00000036738 ENSRNOT00000038938 ENSRNOT00000027121 ENSRNOT00000000889 ENSRNOT00000068658 ENSRNOT00000019865 ENSRNOT00000008172 ENSRNOT00000007467 ENSRNOT00000044407 ENSRNOT00000016833 ENSRNOT00000018858 ENSRNOT00000027360 ENSRNOT00000023762 ENSRNOT00000028179

Gene Information

Description Not Available

Gene Symbol:   SAP18
Gene Name:   Protein RGD1561590; RCG23529, isoform CRA_a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000020771
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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