PrimePCR™ Probe Assay: Olr1702, Rat

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032547
Assay Design:   exonic
Chromosome Location:   20:1108678-1108824question
Amplicon Length:   117
Splice Variants Targeted:   ENSRNOT00000018511 ENSRNOT00000066489 ENSRNOT00000074544 ENSRNOT00000023089 ENSRNOT00000016887 ENSRNOT00000045300 ENSRNOT00000050789 ENSRNOT00000035034 ENSRNOT00000009931 ENSRNOT00000068077 ENSRNOT00000004365 ENSRNOT00000024618 ENSRNOT00000000474 ENSRNOT00000055249 ENSRNOT00000021298 ENSRNOT00000040740 ENSRNOT00000048253 ENSRNOT00000073707 ENSRNOT00000073398 ENSRNOT00000041697 ENSRNOT00000025815

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr1702
Gene Name:   Olfactory receptor Olr1702
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000050738
Entrez:   294157
UniGene:   Rn.124753
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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