PrimePCR™ Probe Assay: Nme8, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032409
Assay Design:   exonic
Chromosome Location:   17:56124764-56140925question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000066673 ENSRNOT00000059202 ENSRNOT00000059201 ENSRNOT00000015033

Gene Information

a protein incorporated into the fibrous sheath of mature sperm tails; may be required for sperm maturation in the testis and/or epididymis [RGD, Feb 2006]

Gene Symbol:   Nme8
Gene Name:   Thioredoxin domain-containing protein 3
Aliases:   Not Available
RefSeq:   NM_198792
Ensembl:   ENSRNOG00000027604
Entrez:   364729
UniGene:   Rn.107066
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.993000
y-intercept 37.810000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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