PrimePCR™ Probe Assay: RGD1560584, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032371
Assay Design:   exonic
Chromosome Location:   4:157238582-157238726question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000009426 ENSRNOT00000047163 ENSRNOT00000046984 ENSRNOT00000017635 ENSRNOT00000046053 ENSRNOT00000030194 ENSRNOT00000049646 ENSRNOT00000048281 ENSRNOT00000035711 ENSRNOT00000048766 ENSRNOT00000065546 ENSRNOT00000048758 ENSRNOT00000043147 ENSRNOT00000058640 ENSRNOT00000046785 ENSRNOT00000040376 ENSRNOT00000022114 ENSRNOT00000040905 ENSRNOT00000031497 ENSRNOT00000041628 ENSRNOT00000045504 ENSRNOT00000040676 ENSRNOT00000036049 ENSRNOT00000044787 ENSRNOT00000046874 ENSRNOT00000046626 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RGD1560584
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000038437
Entrez:   500154
UniGene:   Not Available
Chromosome Mapping:   4q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 37.310000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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