PrimePCR™ Probe Assay: Krtap3-3, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032238
Assay Design:   exonic
Chromosome Location:   10:87264894-87265003question
Amplicon Length:   80
Splice Variants Targeted:   ENSRNOT00000061402 ENSRNOT00000008989 ENSRNOT00000008752 ENSRNOT00000070986 ENSRNOT00000001211 ENSRNOT00000005052 ENSRNOT00000068044 ENSRNOT00000034910 ENSRNOT00000015670 ENSRNOT00000001503 ENSRNOT00000026336 ENSRNOT00000038612 ENSRNOT00000014118 ENSRNOT00000014104 ENSRNOT00000026263 ENSRNOT00000024185 ENSRNOT00000039983 ENSRNOT00000045189

Gene Information

Description Not Available

Gene Symbol:   Krtap3-3
Gene Name:   Protein Krtap3-3; Protein Krtap3-3l1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045678
Entrez:   363678
UniGene:   Not Available
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 37.460000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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