PrimePCR™ Probe Assay: Olr845, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: Olr845, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Olr845, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0032128
Assay Design:   exonic
Chromosome Location:   5:73681990-73682099question
Amplicon Length:   80
Splice Variants Targeted:   ENSRNOT00000066032 ENSRNOT00000059426 ENSRNOT00000061933 ENSRNOT00000071699 ENSRNOT00000071002 ENSRNOT00000021689 ENSRNOT00000013038 ENSRNOT00000008475 ENSRNOT00000020940 ENSRNOT00000075605 ENSRNOT00000052167 ENSRNOT00000070882 ENSRNOT00000072171 ENSRNOT00000050490 ENSRNOT00000073293 ENSRNOT00000041134 ENSRNOT00000051234 ENSRNOT00000047909 ENSRNOT00000052426 ENSRNOT00000039903 ENSRNOT00000072957 ENSRNOT00000075727 ENSRNOT00000074807 ENSRNOT00000046638 ENSRNOT00000074390 ENSRNOT00000028575 ENSRNO

Gene Information

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Gene Symbol:   Olr845
Gene Name:   Olfactory receptor Olr845
Aliases:   Not Available
RefSeq:   NM_001000404
Ensembl:   ENSRNOG00000048481
Entrez:   298046
Chromosome Mapping:   5q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.310000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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