PrimePCR™ Probe Assay: Hist1h2bh, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031805
Assay Design:   exonic
Chromosome Location:   17:58950319-58950415question
Amplicon Length:   67
Splice Variants Targeted:   ENSRNOT00000015878 ENSRNOT00000032047 ENSRNOT00000025878 ENSRNOT00000022358 ENSRNOT00000028779 ENSRNOT00000005267 ENSRNOT00000017294 ENSRNOT00000055536 ENSRNOT00000064555 ENSRNOT00000066932 ENSRNOT00000025142 ENSRNOT00000002928 ENSRNOT00000024281 ENSRNOT00000026897 ENSRNOT00000054753 ENSRNOT00000072195 ENSRNOT00000071688 ENSRNOT00000041423 ENSRNOT00000048465

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bh
Gene Name:   Histone H2B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045750
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.050000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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