PrimePCR™ Probe Assay: Tmem158, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031738
Assay Design:   exonic
Chromosome Location:   8:131498186-131498294question
Amplicon Length:   79
Splice Variants Targeted:   ENSRNOT00000015114 ENSRNOT00000035906 ENSRNOT00000007181 ENSRNOT00000068322 ENSRNOT00000023789 ENSRNOT00000006300

Gene Information

receptor that binds brain injury-derived neurotrophic peptide; abolishes the protective effect of brain injury-derived neurotrophic peptide [RGD, Feb 2006]

Gene Symbol:   Tmem158
Gene Name:   Transmembrane protein 158
Aliases:   Not Available
RefSeq:   NM_057212
Ensembl:   ENSRNOG00000004757
Entrez:   117582
UniGene:   Rn.4294
Chromosome Mapping:   8q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.150000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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