PrimePCR™ Probe Assay: Hist1h2bcl1, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031514
Assay Design:   exonic
Chromosome Location:   17:58731053-58731281question
Amplicon Length:   199
Splice Variants Targeted:   ENSRNOT00000021405 ENSRNOT00000073712 ENSRNOT00000038175 ENSRNOT00000067060 ENSRNOT00000022432 ENSRNOT00000028779 ENSRNOT00000001387 ENSRNOT00000073931 ENSRNOT00000047596 ENSRNOT00000000662 ENSRNOT00000037877 ENSRNOT00000004406 ENSRNOT00000016423 ENSRNOT00000015963 ENSRNOT00000042070 ENSRNOT00000024581 ENSRNOT00000046129 ENSRNOT00000035512 ENSRNOT00000066190 ENSRNOT00000041187 ENSRNOT00000022630 ENSRNOT00000072195 ENSRNOT00000030198 ENSRNOT00000075811 ENSRNOT00000072293 ENSRNOT00000071688 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Hist1h2bcl1
Gene Name:   Histone H2B
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046693
Entrez:   680403
UniGene:   Not Available
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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