PrimePCR™ Probe Assay: RPL21, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RPL21, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RPL21, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031479
Assay Design:   exonic
Chromosome Location:   3:98528769-98528889question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000031915 ENSRNOT00000015795 ENSRNOT00000001265 ENSRNOT00000002790 ENSRNOT00000038139 ENSRNOT00000006796 ENSRNOT00000016736 ENSRNOT00000021161 ENSRNOT00000058884 ENSRNOT00000013161 ENSRNOT00000039709 ENSRNOT00000024999 ENSRNOT00000012902 ENSRNOT00000015571 ENSRNOT00000016479 ENSRNOT00000021265 ENSRNOT00000057136 ENSRNOT00000027783 ENSRNOT00000021876 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000071210 ENSRNOT00000049138 ENSRNOT00000048673 ENSRNOT00000058595 ENSRNOT00000048420 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   RPL21
Gene Name:   60S ribosomal protein L21; 60S ribosomal protein L21; Protein LOC100360604; RCG42727, isoform CRA_a; Ribosomal protein L21
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049848
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.110000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download