PrimePCR™ Probe Assay: Sftpb, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031431
Assay Design:   exonic
Chromosome Location:   4:164942126-164942905question
Amplicon Length:   96
Splice Variants Targeted:   ENSRNOT00000014505

Gene Information

acts to lower surface tension in lung alveoli [RGD, Feb 2006]

Gene Symbol:   Sftpb
Gene Name:   Pulmonary surfactant-associated protein B precursor
Aliases:   Not Available
RefSeq:   NM_138842
Ensembl:   ENSRNOG00000010761
Entrez:   192155
UniGene:   Rn.1952
Chromosome Mapping:   4q33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.640000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download