PrimePCR™ Probe Assay: Fgfr2, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031319
Assay Design:   exonic
Chromosome Location:   1:207684093-207693845question
Amplicon Length:   93
Splice Variants Targeted:   ENSRNOT00000015401 ENSRNOT00000068183 ENSRNOT00000022253 ENSRNOT00000065448 ENSRNOT00000022331

Gene Information

may play a role in mesodermal cell differentiation [RGD, Feb 2006]

Gene Symbol:   Fgfr2
Gene Name:   Fibroblast growth factor receptor 2 isoform a
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000016374
Entrez:   25022
Chromosome Mapping:   1q37

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.210000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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