PrimePCR™ Probe Assay: Hspb7, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0031154
Assay Design:   exonic
Chromosome Location:   5:163691570-163691690question
Amplicon Length:   91
Splice Variants Targeted:   ENSRNOT00000051317

Gene Information

heat shock protein that binds alpha-filamin [RGD, Feb 2006]

Gene Symbol:   Hspb7
Gene Name:   Heat shock protein beta-7
Aliases:   Not Available
RefSeq:   NM_031607
Ensembl:   ENSRNOG00000029079
Entrez:   50565
UniGene:   Rn.21809
Chromosome Mapping:   5q36

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.820000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download