PrimePCR™ Probe Assay: Mafb, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0030822
Assay Design:   exonic
Chromosome Location:   3:162574255-162574369question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000021452

Gene Information

nuclear bZip transcription factor; involved in the late stages of chondrocyte development [RGD, Feb 2006]

Gene Symbol:   Mafb
Gene Name:   Transcription factor MafB
Aliases:   Not Available
RefSeq:   NM_019316
Ensembl:   ENSRNOG00000016037
Entrez:   54264
UniGene:   Rn.10725
Chromosome Mapping:   3q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.340000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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