PrimePCR™ Probe Assay: Top2a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0030155
Assay Design:   exonic
Chromosome Location:   10:86700090-86700207question
Amplicon Length:   88
Splice Variants Targeted:   ENSRNOT00000050725

Gene Information

DNA topoisomerase, a nuclear enzyme that can alter the topologic state of DNA during transcription [RGD, Feb 2006]

Gene Symbol:   Top2a
Gene Name:   DNA topoisomerase 2-alpha
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000032404
Entrez:   360243
UniGene:   Rn.90996
Chromosome Mapping:   10q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.410000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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