PrimePCR™ Probe Assay: Timm44, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0029941
Assay Design:   exonic
Chromosome Location:   12:4676075-4676214question
Amplicon Length:   110
Splice Variants Targeted:   ENSRNOT00000001409

Gene Information

mouse homolog is a translocase of the inner mitochondrial membrane; can be up-regulated in hyperglycemic states [RGD, Feb 2006]

Gene Symbol:   Timm44
Gene Name:   Mitochondrial import inner membrane translocase subunit TIM44
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000001058
Entrez:   29635
UniGene:   Rn.10801
Chromosome Mapping:   12p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.930000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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