PrimePCR™ Probe Assay: Unc13b, Rat

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Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0029308
Assay Design:   exonic
Chromosome Location:   5:63218201-63218298question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000025162 ENSRNOT00000011863 ENSRNOT00000043216 ENSRNOT00000067281 ENSRNOT00000064755

Gene Information

brain specific protein that may have a role in neurotransmitter release [RGD, Feb 2006]

Gene Symbol:   Unc13b
Gene Name:   Protein unc-13 homolog B isoform u
Aliases:   Not Available
RefSeq:   NM_001042579 NM_022862
Ensembl:   ENSRNOG00000008237
Entrez:   64830
UniGene:   Rn.10127
Chromosome Mapping:   5q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.720000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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