PrimePCR™ Probe Assay: Dusp14, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0028388
Assay Design:   exonic
Chromosome Location:   10:71278319-71278436question
Amplicon Length:   88
Splice Variants Targeted:   ENSRNOT00000036094 ENSRNOT00000048687 ENSRNOT00000043148 ENSRNOT00000005684 ENSRNOT00000042276 ENSRNOT00000052029 ENSRNOT00000074234 ENSRNOT00000016740 ENSRNOT00000074141

Gene Information

Description Not Available

Gene Symbol:   Dusp14
Gene Name:   Dual specificity phosphatase 14
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030091
Entrez:   360580
Chromosome Mapping:   10q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.980000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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