PrimePCR™ Probe Assay: Rps14, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rps14, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rps14, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0027885
Assay Design:   exonic
Chromosome Location:   18:55280918-55281539question
Amplicon Length:   61
Splice Variants Targeted:   ENSRNOT00000000060 ENSRNOT00000040464 ENSRNOT00000059501 ENSRNOT00000066864 ENSRNOT00000003633 ENSRNOT00000010614 ENSRNOT00000013809 ENSRNOT00000067558 ENSRNOT00000006554 ENSRNOT00000041688 ENSRNOT00000020603 ENSRNOT00000005839 ENSRNOT00000026569 ENSRNOT00000060767 ENSRNOT00000026482 ENSRNOT00000036330 ENSRNOT00000021359 ENSRNOT00000021379 ENSRNOT00000035758 ENSRNOT00000042949

Gene Information

structural component of the 40S subunit of the ribosome, the organelle responsible for protein synthesis [RGD, Feb 2006]

Gene Symbol:   Rps14
Gene Name:   40S ribosomal protein S14
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000018774
Entrez:   29284
Chromosome Mapping:   18q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.650000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download