PrimePCR™ Probe Assay: Rplp0, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Rplp0, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Rplp0, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0027073
Assay Design:   exonic
Chromosome Location:   12:48589001-48589127question
Amplicon Length:   97
Splice Variants Targeted:   ENSRNOT00000031963 ENSRNOT00000060648 ENSRNOT00000010648 ENSRNOT00000013044 ENSRNOT00000001518 ENSRNOT00000064695 ENSRNOT00000040909

Gene Information

member of the ribosomal protein family; has similarity to other P ribosomal proteins over the carboxyl terminal sequence [RGD, Feb 2006]

Gene Symbol:   Rplp0
Gene Name:   60S acidic ribosomal protein P0
Aliases:   Not Available
RefSeq:   NM_022402
Ensembl:   ENSRNOG00000001148
Entrez:   64205
Chromosome Mapping:   12q16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 1.000000
y-intercept 35.990000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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