PrimePCR™ Probe Assay: Nes, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0026391
Assay Design:   exonic
Chromosome Location:   2:206753975-206754085question
Amplicon Length:   81
Splice Variants Targeted:   ENSRNOT00000066940 ENSRNOT00000067979 ENSRNOT00000015705 ENSRNOT00000044613 ENSRNOT00000070881 ENSRNOT00000073111 ENSRNOT00000025314 ENSRNOT00000025384 ENSRNOT00000023116 ENSRNOT00000051927

Gene Information

intermediate filament protein specifically expressed in neuroepithelial stem cells; may play a role in neural cell differentiation [RGD, Feb 2006]

Gene Symbol:   Nes
Gene Name:   Nestin
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000018681
Entrez:   25491
UniGene:   Rn.9701
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.920000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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