PrimePCR™ Probe Assay: Taar9, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $196.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025761
Assay Design:   exonic
Chromosome Location:   1:23799839-23799934question
Amplicon Length:   66
Splice Variants Targeted:   ENSRNOT00000038523

Gene Information

neurotransmitter/neuromodulator of trace amines; responsible for psychiatric disorders, depression and migraine [RGD, Feb 2006]

Gene Symbol:   Taar9
Gene Name:   Trace amine-associated receptor 9
Aliases:   Not Available
RefSeq:   NM_175602
Ensembl:   ENSRNOG00000026096
Entrez:   319107
UniGene:   Rn.138162
Chromosome Mapping:   1p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 37.520000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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