PrimePCR™ Probe Assay: Tpm3, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0025017
Assay Design:   exonic
Chromosome Location:   2:208870822-208871008question
Amplicon Length:   64
Splice Variants Targeted:   ENSRNOT00000021073 ENSRNOT00000017339 ENSRNOT00000005073 ENSRNOT00000000655 ENSRNOT00000013286 ENSRNOT00000045270 ENSRNOT00000020669 ENSRNOT00000034672 ENSRNOT00000014249 ENSRNOT00000014388 ENSRNOT00000038160 ENSRNOT00000023567 ENSRNOT00000023605 ENSRNOT00000029076

Gene Information

rod-like protein associated with actin filament in nonmuscle cells; may mediate the stability of actin filaments; may play a role in the regulation of cell transformation, cytokinesis, motility, and morphogenesis [RGD, Feb 2006]

Gene Symbol:   Tpm3
Gene Name:   Tropomyosin alpha-3 chain
Aliases:   Not Available
RefSeq:   NM_173111 NM_057208
Ensembl:   ENSRNOG00000017441
Entrez:   117557
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 35.550000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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