PrimePCR™ Probe Assay: Stat5a, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024848
Assay Design:   exonic
Chromosome Location:   10:88582770-88582873question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000026662

Gene Information

member of the STAT family of transcription factors; may mediate the biological actions of several interleukin factors (IL2, IL3, IL7) and growth hormones; may be associated with mammary gland function [RGD, Feb 2006]

Gene Symbol:   Stat5a
Gene Name:   Signal transducer and activator of transcription 5A
Aliases:   Not Available
RefSeq:   NM_017064
Ensembl:   ENSRNOG00000019496
Entrez:   24918
UniGene:   Rn.154399,Rn.228580
Chromosome Mapping:   10q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.370000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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