PrimePCR™ Probe Assay: Bax, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024456
Assay Design:   exonic
Chromosome Location:   1:102530751-102530967question
Amplicon Length:   187
Splice Variants Targeted:   ENSRNOT00000017730 ENSRNOT00000028328

Gene Information

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD, Feb 2006]

Gene Symbol:   Bax
Gene Name:   Apoptosis regulator BAX
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000020876
Entrez:   24887
UniGene:   Rn.10668,Rn.232358
Chromosome Mapping:   1q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.500000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download