PrimePCR™ Probe Assay: Echs1, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: Echs1, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: Echs1, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0024340
Assay Design:   exonic
Chromosome Location:   1:219484873-219484965question
Amplicon Length:   63
Splice Variants Targeted:   ENSRNOT00000048854 ENSRNOT00000044081 ENSRNOT00000045150 ENSRNOT00000019844 ENSRNOT00000036744 ENSRNOT00000024736 ENSRNOT00000032152 ENSRNOT00000009436 ENSRNOT00000017224 ENSRNOT00000024364 ENSRNOT00000031904 ENSRNOT00000023010 ENSRNOT00000028593 ENSRNOT00000071574 ENSRNOT00000035770 ENSRNOT00000049430 ENSRNOT00000025446 ENSRNOT00000025664

Gene Information

catalyzes the conversion of (3S)-3-hydroxyacyl-CoA to trans-2(or 3)-enoyl-CoA and H2O in fatty acid beta-oxidation [RGD, Feb 2006]

Gene Symbol:   Echs1
Gene Name:   Enoyl-CoA hydratase, mitochondrial
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000018522
Entrez:   140547
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.610000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download