This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: Cyp8b1, Rat
PrimePCR™ Template for Probe Assay: Cyp8b1, Rat
member of the cytochrome P450 enzyme superfamily; endoplasmic reticulum membrane protein; plays a role in sterol metabolism; human homolog is unique among cytochrome P450 genes in that it is intronless [RGD, Feb 2006]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.