PrimePCR™ Probe Assay: Dync1h1, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCEP0023368
Assay Design:   exonic
Chromosome Location:   6:144110091-144110209question
Amplicon Length:   89
Splice Variants Targeted:   ENSRNOT00000047160 ENSRNOT00000073950 ENSRNOT00000009377

Gene Information

cytoplasmic motor protein that propels membranous organelles towards (-) ends of polarized microtubules using ATP hydrolysis; may be responsible for retrograde axonal transport and (-) end-directed organelle movements [RGD, Feb 2006]

Gene Symbol:   Dync1h1
Gene Name:   Cytoplasmic dynein 1 heavy chain 1
Aliases:   Not Available
RefSeq:   NM_019226
Ensembl:   ENSRNOG00000006178
Entrez:   29489
UniGene:   Rn.11027
Chromosome Mapping:   6q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 37.510000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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